Effects of 2-Deoxy-D-glucose on DNA Repair and Mutagenesis in UV-irradiated Yeast
نویسندگان
چکیده
منابع مشابه
Effects of 2-deoxy-D-glucose on DNA repair and mutagenesis in UV-irradiated yeast.
We have studied the effects of 2-deoxy-D-Glucose (2-DG) on the recovery of potentially lethal damage (PLDR), repair of chromosomal DNA, cyclobutane pyrimidine dimers (CPDs), reverse mutation and gene-conversion in UVC (254 nm) irradiated yeast. As studied by pulsed-field gel electrophoresis, post-irradiation chromosomal DNA repair kinetics in a phosphate buffer (PB) with 10 mM glucose (G) was b...
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Many fundamental biological processes have been elucidated by the isolation and analysis of mutants that are defective in such processes. Therefore, the methods to generate mutants are of great importance in model organisms. This unit describes two protocols for mutagenesis of yeast-using ethyl methanesulfate (EMS) and ultraviolet (UV) light. Each of these methods has been used successfully for...
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ژورنال
عنوان ژورنال: Journal of Radiation Research
سال: 2001
ISSN: 0449-3060
DOI: 10.1269/jrr.42.285